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KMID : 0894520120160020077
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Development & Reproduction
2012 Volume.16 No. 2 p.77 ~ p.85
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Assessment of Sperm Activity of Black Porgy(Acanthopagrus schlegeli) Acclimated in Freshwater on Cryopreservation Condition
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Jeong Min-Hwan
Lim Han-Kyu
Do Yong-Hyun
Kim Jong-Hyun
Son Maeng-Hyun
Chang Young-Jin
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Abstract
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Various cryoprotective agents (CPA) were tested to establish the best conditions for the cryopreservation of sperm from black porgy Acanthopagrus schlegeli acclimated and raised in freshwater (BFW). Survival rates of frozen/thawed sperm from BFW were higher in the order of dimethy sulfoxide (DMSO), glycerol, ethylene glycol (EG) and methanol. Sperm motility was higher in the order of glycerol, DMSO, EG and methanol. These effects were the same in thawed sperm from black porgy raised in seawater (BSW). Thus, optimum CPA for sperm cryopreservation of BFW and BSW were DMSO and glycerol where the highest survival rates and sperm motility were found at the concentration of 10%. In particular, the survival rates and motility of thawed sperm from BFW and BSW after cryopreservation using 10% DMSO were better than when cryopreserved using 10% glycerol. On the other hand, for the thawed sperm from both BFW and BSW, the longer the preservation period was, the lower the survival rates and sperm motility were. Notably, the higher the concentration of CPA was, the lower the survival rates and sperm motility were.
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KEYWORD
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Acanthopagrus schlegeli, Freshwater acclimation, Sperm activity, Cryoprotective agents, Cryopreservation
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